Direct detection of Borrelia burgdorferi in Ixodes scapularis (Acari : Ixodidae) nymphs by hybridization to ribosomal RNA

Authors
Danishefsky, K Ramachandra, N Coleman, J Schwartz, I
Citation
K. Danishefsky et al., Direct detection of Borrelia burgdorferi in Ixodes scapularis (Acari : Ixodidae) nymphs by hybridization to ribosomal RNA, J MED ENT, 37(1), 2000, pp. 102-107
Citations number
15
Categorie Soggetti
Entomology/Pest Control
Journal title
JOURNAL OF MEDICAL ENTOMOLOGY
ISSN journal
00222585 → ACNP
Volume
37
Issue
1
Year of publication
2000
Pages
102 - 107
Database
ISI
SICI code
0022-2585(200001)37:1<102:DDOBBI>2.0.ZU;2-3
Abstract
A method for direct detection of Borrelia burgdorferi Johnson, Schmid, Hyde , Steigerwalt & Brenner has been developed. Cells are lysed to facilitate r elease of ribosomal RNA. Lysates are filtered onto nylon membranes that are hybridized with probes specific for sequences in B. burgdorferi 23S rRNA. The technique is rapid and does not require any enzymatic amplification ste ps. With the use of a cocktail containing five different proles, approximat e to 1,000 organisms could be detected. The assay was successfully applied to direct detection of B. burgdorferi in Ixodes scapularis Say nymphs.