Spatiotemporal induction pattens of cytokine and related immune signal molecule mRNAs in response to intrastriatal injection of lipopolysaccharide (vol. 106, pg. 114, 2000)

The brain's response to a direct immune challenge was examined by in situ h ybridization histochemistry. Lipopolysaccharide (bacterial endotoxin) injec ted acutely into rat striatum induced mRNA expression for inhibitory factor kappa B alpha, interleukin (IL)-1 beta, tumor necrosis factor-alpha, IL-6, IL-12, p35, inducible nitric oxide synthase, IL-1 receptor antagonist, and the type 1 IL-1 receptor. Expression patterns were evaluated at select tim e points ranging from 15 min to 3 days post-injection. Rats injected with v ehicle alone were used to control for mechanical effects. Following lipopol ysaccharide administration, a wave of mRNA induction within brain parenchym a radiated outward from the injection site, generally peaking in intensity at the 16-h time point. The individual profiles of cytokine mRNA induction patterns reveal that the brain's immune response to local inflammatory stim ulation is quite elaborate and in many ways resembles the progression of cy tokine induction customary of localized inflammation in peripheral tissues. (C) 2000 Elsevier Science B.V. All rights reserved.