3 APPROACHES TO ENANTIOMER SEPARATION OF BETA-ADRENERGIC ANTAGONISTS BY CAPILLARY ELECTROCHROMATOGRAPHY

Three different capillary electrochromatographic methods for the enant iomer separation of beta-adrenergic antagonists (acebutolol, alprenolo l, atenolol, metoprolol, pindolol, prenalterol, and propranolol) were applied using different cyclodextrins (beta-cyclodextrin, carboxymethy l-beta-cyclodextrin and dimethyl-beta-cyclodextrin) added to the elect rolyte, a cross-linked protein-gel (cellobiohydrolase I) and a molecul arly imprinted ((R)-enantiomer of propranolol) super-porous polymer as chiral selectors. Through use of these different separation strategie s, all the beta-adrenergic antagonists studied could be resolved into their enantiomers, although the three methods were carried out without extensive optimization. The protein and molecularly imprinted phases gave the highest selectivities whereas employing cyclodextrins resulte d in the highest separation efficiency. Proteins and cyclodextrins are primarily natural products, albeit the cyclodextrins can be derivatiz ed. In contrast, the molecularly imprinted chiral stationary phase can be highly customized when produced.