CHIRAL INTERACTIONS IN CAPILLARY ZONE ELECTROPHORESIS - COMPUTER-SIMULATION AND COMPARISON WITH EXPERIMENT

Chiral interaction in capillary electrophoresis can be modeled using p K values, mobilities of analytes, and their formation constants with t he chiral selector. An existing steady-state simulation program for CE (HPCESIM) was recently extended with a chiral submenu involving the c hiral parameters listed above. These were experimentally determined in both our laboratories for mandelic acid and terbutaline using hydroxy propylated beta-cyclodextrin as chiral selector. A comparison was made between both sets of parameters and between experimental electrophero grams and those obtained from simulation. Error analysis of the result s indicate the sensitivity of the obtained results.