DETERMINATION OF THE ENANTIOMERIC PURITY OF N-PROPIONYL-6,7-DIMETHOXY-2-AMINOTETRALIN BY CYCLODEXTRIN-MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY

N-Propionyl-6,7-dimethoxy-2-aminotetralin (Z12231A), a useful intermed iate in the synthesis of dopaminergic agonists, was resolved into its enantiomers by high-performance capillary electrophoresis. Cyclodextri n-modified micellar electrokinetic chromatography was employed with a combination of chiral selectors both in the aqueous phase (a hydroxyal kyl-beta-cyclodextrin) and in the micellar phase (sodium taurodeoxycho late). The absolute amount of the two chiral selectors as well as thei r ratio were found to be the most critical parameters in order to opti mize separation. A resolution factor > 3.5 was obtained, allowing a hi gh amount of solute to be loaded in order to improve the detection lim it. The reproducibility of the assay was also evaluated.