Objective: To examine the effect of various combinations of beverages on hy
dration status in healthy free-living adult males.
Methods: In a counterbalanced, crossover manner, 18 healthy adult males age
s 24 to 39, on four separate occasions, consumed water or water plus Varyin
g combinations of beverages. Clinical guidelines were used to determine the
fluid allowance for each subject. The beverages were carbonated, caffeinat
ed caloric and non-caloric colas and coffee. Ten of the 18 subjects consume
d water and carbonated, non-caffeinated, citrus soft drink during a fifth t
rial. Body weight, urine and blood assays were measured before and after ea
ch treatment.
Results: Slight body weight loss was observed on all treatments, with an av
erage of 0.30% for all treatments. No differences (p>0.05) among treatments
were found for body weight changes or any of the biochemical assays. Bioch
emical assays conducted on first voids and 24-hour urines included electrol
ytes, creatine, osmolality and specific gravity. Blood samples were analyze
d for hemoglobin, hematocrit, electrolytes, osmolality, urea nitrogen, crea
tinine and protein.
Conclusions: This preliminary study found no significant differences in the
effect of various combinations of beverages on hydration status of healthy
adult males. Advising people to disregard caffeinated beverages as part of
the daily fluid intake is not substantiated by the results of this study.
The across-treatment weight loss observed, when combined with data on fluid
-disease relationships, suggests that optimal fluid intake may be higher th
an common recommendations. Further research is needed to confirm these resu
lts and to explore optimal fluid intake for healthy individuals.