Jk. Park et al., Cerivastatin prevents angiotensin II-induced renal injury independent of blood pressure- and cholesterol-lowering effects, KIDNEY INT, 58(4), 2000, pp. 1420-1430
Background. Statins are effective in prevention of endorgan damage; however
, the benefits cannot be fully explained on the basis of cholesterol reduct
ion. We used an angiotensin II (Ang II)-dependent model to test the hypothe
sis that cerivastatin prevents leukocyte adhesion and infiltration, inducti
on of inducible nitric oxide synthase (iNOS), and ameliorates endorgan dama
ge.
Methods. We analyzed intracellular targets, such as mitogen activated prote
in kinase and transcription factor (nuclear factor-kappa B and activator pr
otein-1) activation. We used immunohistochemistry, immunocytochemistry, ele
ctrophoretic mobility shift assays, and enzyme-linked immunosorbent assay t
echniques. We treated rats transgenic for human renin and angiotensinogen (
dTGR) chronically from week 4 to 7 with cerivastatin (0.5 mg/kg by gavage).
Results. Untreated dTGR developed hypertension, cardiac hypertrophy, and re
nal damage, with a 100-fold increased albuminuria and focal cortical necros
is. dTGR mortality at the age of seven weeks was 45%. Immunohistochemistry
showed increased iNOS expression in the endothelium and media of small vess
els, infiltrating cells. afferent arterioles, and glomeruli of dTGR, which
was greater in cortex than medulla. Phosphorylated extracellular signal reg
ulated kinase (p-ERK) was increased in dTGR: nuclear factor-kappa B and act
ivator protein-1 were both activated. Cerivastatin decreased systolic blood
pressure compared with untreated dTGR (147 +/- 14 vs. 201 +/- 6 mm Hg, P <
0.001). Albuminuria was reduced by 60% (P = 0.001), and creatinine was low
ered (0.45 +/- 0.01 vs. 0.68 +/- 0.05 mg/dL, P = 0.003); however, cholester
ol was not reduced. Intercellular adhesion molecule-1 and vascular cell adh
esion molecule-1 expression was diminished, while neutrophil and monocyte i
nfiltration in the kidney was markedly reduced. ERK phosphorylation and tra
nscription factor activation were reduced. In addition, in vitro incubation
of vascular smooth muscle cells with cerivastatin (0.5 mu mol/L) almost co
mpletely prevented the Ang II-induced ERK phosphorylation.
Conclusion. Cerivastatin reduced inflammation, cell proliferation, and iNOS
induction, which led to a reduction in cellular damage. Our findings sugge
st that 3-hydroxy-3-methylglutaryl coenzyme (HMG-CoA) reductase inhibition
ameliorates Ang II-induced end-organ damage. We suggest that these effects
were independent of cholesterol.