Determination of the phospholipid/lipophilic compounds ratio in liposomes by thin-layer chromatography scanning densitometry

The determination of the ratio of phospholipid/lipophilic compounds in lipo somes was achieved after thin-layer chromatography (TLC) by measuring the s pot intensities of dipalmitoyl phosphatidylcholine and the lipophilic compo und. The liposome components under study were separated on one TLC plate, d eveloped in two steps, and detected after charring the plate with specific visualization reagents. The method shows good reproducibility and provides a simple way to quantify the level of lipophilic compound incorporated in t he liposome bilayer.