Hts. Boschker et al., Limited coupling of macrophyte production and bacterial carbon cycling in the sediments of Zostera spp. meadows, MAR ECOL-PR, 203, 2000, pp. 181-189
Two approaches were used to study coupling between seagrass production and
bacterial processes in the rhizosphere. In the first approach, stable carbo
n-isotope ratios of bacteria, sediment organic matter and plants were deter
mined to infer sources of organic carbon used by bacteria in the sediments
of 4 European Zostera marina and 2 Z. noltii meadows. Bacterial isotope rat
ios were derived from bacteria-specific polar lipid-derived fatty acids (PL
FA), mainly methyl-branched i15:0 and a15:0. Bacterial delta(13)C ratios in
the sediment from both vegetated and unvegetated sites were mostly similar
and did not resemble Z. marina ratios, suggesting that seagrass material w
as of limited importance as a bacterial carbon source. Bacterial ratios wer
e in most cases similar to benthic macroalgae and did correlate well with r
atios of diatom biomarkers. Sediment organic matter inside the meadows had
delta(13)C ratios similar to those of nearby unvegetated sites, and ratios
were clearly different from the material produced by seagrasses, indicating
that little seagrass material accumulated. Results from the 2 Z, noltii si
tes were less conclusive, as there was no clear difference in delta(13)C ra
tios between the potential source materials. In addition, bacterial delta(1
3)C ratios were highly variable at one Z, noltii site. In the second approa
ch, cultured Z, marina was labeled with C-13-bicarbonate to study the short
-term transfer of label from plants to bacteria in the rhizosphere. However
, no label was detected in bacterial PLFA after 20 h of incubation. In conc
lusion, a close coupling between macrophyte production and bacterial carbon
cycling could not be detected in the sediment of Z. marina meadows, and be
nthic production by algae was probably the main carbon source for bacterial
growth.