Distal chromosome 9p contains a locus that, when deleted, is a cause of 46,
XY gonadal dysgenesis in the absence of extragenital anomalies. This locus
might account for the frequently observed cases of 46,XY pure gonadal dysge
nesis who do not harbor mutations in SRY, the sex master regulator gene fou
nd in mammalian species. The genomic organization of 9p positional candidat
e genes is currently being studied and mutational screens are ongoing. Amon
g other positional candidates, including two additional doublosex-related g
enes, the evidence to support a role for the gene DMRT1 in vertebrate male
sexual development is accumulating. Although formal proof of the requiremen
t of DMRT1 in gonadal sex fate choice has not been obtained so far, the par
ticular interest in this gene and perhaps other doublesex-related genes ide
ntified in vertebrates lies in that they may provide an entry point to a co
nserved mechanism of sex determination across animal phyla. We discuss rece
nt results and emerging views on the genetics of sex determination, while s
tressing that the majority of cases of 46,XY gonadal dysgenesis remain unex
plained. The latter is likely to be efficiently addressed by positional clo
ning efforts, particularly by considering the wealth of sequence data provi
ded by the Human Genome Project. (C) 2000 Academic Press.