Elimination of early Salmonella enteritidis infection after treatment withcompetitive-exclusion culture and enrofloxacin in experimentally infected chicks
Kh. Seo et al., Elimination of early Salmonella enteritidis infection after treatment withcompetitive-exclusion culture and enrofloxacin in experimentally infected chicks, POULTRY SCI, 79(10), 2000, pp. 1408-1413
The effect of normal avian gut flora (NAGF) and enrofloxacin administration
on the early infection of young chicks by Salmonella enteritidis (SE) was
determined using day-old White Leghorn chicks. Day-old chicks were divided
into two groups, untreated control:and :NAGF-treated, and then infected wit
h 10(6) cfu of SE per chick by oral gavage. The untreated, infected chicks
were further divided into two groups and were either left untreated or medi
cated with a regimen of 10 mg/kg of enrofloxacin in drinking water daily fo
r 10 d, followed by two doses of NAGF beginning at 10 and 8 wk bf age in Tr
ial 1 and Trial 2, respectively. Liver, spleen, and cecum samples were test
ed for the presence of SE, and immunological responsiveness was investigate
d up to 12 wk of age. Compared with the untreated group, the cecal coloniza
tion of SE was significantly (P < 0.05) decreased in the NAGF-treated group
in Trials 1 and 2. No significant differences in organ infection were obse
rved in the NAGF-treated vs, untreated birds. although a significant effect
of the combined treatment of enrofloxacin treatment and NAGF on the early
infection was not shown in Trial 1, compared with enrofloxacin only or the
untreated group, a significant reduction (P < 0.05) in the number of infect
ed chickens and in the number of SE in the cecal contents was observed at 1
0 wk of age in Trial 2. The enrofloxacin treatment did not increase opportu
nistic colonization by SE due to the use of the antibiotic in either trial.
The plasma and intestinal immunological responses were not significant at
the early age (up to 12 wk) of the birds. The use of enrofloxacin, followed
by NAGF, could aid the elimination of SE from young chicks persistently in
fected at an early age. The combined treatment, compared with enrofloxacin
alone, protected chickens from reinfection by 40%.