Human T053 from the malignant glioma-derived cell lines M059J and M059K has a cancer-associated mutation in exon 8

The protein coding segment of the TP53 genes from the glioma-derived cell l ines M059J and M059K was sequenced. The sequences from both cell lines were identical over 5039 bp, including the gene segment containing exons 2 thro ugh 9, exon 10, and the proximal segment of exon 11. In both cells, the fir st nucleotide of codon 286 (GAA, Glu) is changed to an A (AAA, Lys). Compar ison with the same TP53 segment from the A549 human lung carcinoma cell lin e revealed several differences in intron sequence. (C) 2000 by Radiation Re search Society.