Study on the fluorescence spectra and electrochemical behavior of ZnL2 andMorin with DNA

The interactions of Morin (2', 3, 4', 5, 7-pentahydroxyflavone) and its Zn- complex, ZnL2. 3H(2)O [L = Morin (2'-OH group deprotonated)], with calf thy mus DNA have been studied using fluorimetric and electrochemical methods. Z nL2. 3H(2)O has different spectral characteristics and electrochemical beha vior from that of Morin in the presence of DNA. Increasing fluorescence is seen for ZnL2. 3H(2)O with DNA addition whereas decreased fluorescence is o bserved for Morin. An isosbestic point appears at 560 nm for the DNA-ZnL2. 3H(2)O system with ethidium bromide (EB) addition while no isosbestic point is detectable for Morin. Quenching fluorescence is observed for DNA-EB sys tem when ZnL2. 3H(2)O is added whereas no quenched fluorescence is seen for DNA-EB system with Morin addition. The above results suggest that Morin an d ZnL2. 3H(2)O can both bind to DNA, but the binding mode is different. The complex binds to DNA mainly by intercalation, while Morin binds in a non-i ntercalating mode. The binding constant and binding site sizes are derived from electrochemical methods. For ZnL2. 3H(2)O, the binding constant is 5.0 x 10(4) 1 mol(-1) at 20 degrees C, and the binding site size n(s) is 5. (C ) 2000 Elsevier Science B.V. All rights reserved.