Hy. Sohn et al., Ultradian oscillation of Saccharomyces cerevisiae during aerobic continuous culture: hydrogen sulphide mediates population synchrony, YEAST, 16(13), 2000, pp. 1185-1190
Saccharomyces cerevisiae showed an ultradian respiratory oscillation during
aerobic continuous culture. Analysis of the off-gas revealed that hydrogen
sulphide production also oscillated. Production was first detected at the
onset of low respiration and reached a maximum (1.5 mu M) prior to minimum
respiratory activity. Then H2S concentration fell rapidly to below 0.2 mu M
before the onset of high respiration. Injection of respiratory oscillation
perturbation agents, such as glutathione (50 mu M), NaNO2 (50 mu M) or ace
taldehyde (4.5 mM), transiently increased H2S production above 6 mu M, The
synchronization properties of H2S were analysed to reveal that changes of o
scillation period and amplitude were dependent on H2S concentration in cult
ure. It is concluded that H2S produced during oscillation produces populati
on synchrony by respiratory chain inhibition. Copyright (C) 2000 John Wiley
& Sons, Ltd.