CD95-mediated apoptosis and DNA fragmentation in MS

Objective - To investigate possible associations of soluble CD95 (sCD95) se rum levels and DNA defragmentation with different MS disease stages and act ivities. Methods - Sera of 114 patients were analysed by an ELISA technique for sCD95. In a subgroup of 18 relapsing-remitting MS patients and control s we studied DNA fragmentation by the TUNEL-method in CSF cytospins. Result s - sCD95 was detectable in sera of MS patients, healthy controls and menin gitis patients without significant differences. CSF specimens showed modest amounts of apoptotic cells in MS and controls. Conclusion - We could not d emonstrate an association of MS disease course or activity with the express ion of sCD95 in sera. DNA fragmentation in the CSF was not significantly en hanced compared to controls. Thus the analysed markers of programmed cell d eath appear not suitable to monitor MS disease courses.