Comparison of tear proteins of llamas and cattle

Objective-To analyze and compare contents of the preocular tear films of ll amas and cattle. Animals-40 llamas and 35 cattle. Procedure-Tear pH was determined by use of a pH meter. Total protein concen tration was determined by use of 2 microtiter methods. Tear proteins were s eparated by use of electrophoresis and molecular weights of bands were calc ulated. Western blot immunoassay was used to detect IgA, lactoferrin, trans ferrin, ceruloplasmin, alpha(1)-antitrypsin, alpha(1)-amylase, and alpha(2) -macroglobulin. Enzyme electrophoresis was used to detect proteases. Results-The pH of llama and cattle tears were 8.05 +/- 0.01 and 8.10 +/- 0. 01, respectively. For results of both methods, total protein concentration of llama tears was significantly greater than that of cattle tears. Molecul ar weights of tear protein bands were similar within and between the 2 spec ies, although llama tears had a distinct 13.6-kd band that was not detected in cattle. Lactoferrin, IgA, transferrin, ceruloplasmin, alpha(1)-antitryp sin, alpha(1)-amylase, alpha(2)-macroglobulin, and proteases were detected in both species. Conclusions and Clinical Relevance-llama tears have significantly greater t otal protein concentration than cattle tears, whereas pH is similar between species. Because little variation was detected within species for the numb er and molecular weight of protein bands, pooling of tears for analysis is justified. Results suggest that lactoferrin, ceruloplasmin, transferrin, al pha(1)-antitrypsin, alpha(2)-macroglobulin, alpha(1)-amylase, and IgA are p resent in the tears of llamas and cattle.