Gas and liquid chromatography-mass spectrometry of aldehydic products fromlipid peroxidation

The present report describes the identification of aldehydic products of li pid peroxidation by two chromatographic methods coupled to mass spectrometr y, GC/EI-MS and LC/ESI-IMS, The easy detection of molecular ions in LC/ESI- MS and fragmentation pattern in GC/EI-MS illustrate the complementarity of these two techniques. Several carbonyl compounds, other than the usual repo rted breakdown products of lipid peroxidation, e,g, malondialdehyde and 4-h ydroxynonenal, were identified as 2,4-dinitrophenylhydrazine derivatives in the Fe2+/ascorbate-catalyzed