Structure and calcium-binding properties of Frq1, a novel calcium sensor in the yeast Saccharomyces cerevisiae

The FRQ1 gene is essential for growth of budding yeast and encodes a 190-re sidue, N-myristoylated (myr) calcium-binding protein. Frq1 belongs to the r ecoverin/frequenin branch of the EF-hand superfamily and regulates a yeast phosphatidylinositol 4-kinase isoform. Conformational changes in Frq1 due t o N-myristoylation and Ca2+ binding were assessed by nuclear magnetic reson ance (NMR), fluorescence, and equilibrium Ca2+-binding measurements. For th is purpose, Frq1 and myr-Frq1 were expressed in and purified from Escherich ia coli. At saturation, Frq1 bound three Ca2+ ions at independent sites, wh ich correspond to the second, third, and fourth EF-hand motifs in the prote in. Affinity of the second site (K-d = 10 mu M) was much weaker than that o f the third and fourth sites (K-d = 0.4 mu M). Myr Frq1 bound Ca2+ with a K (d)app of 3 mu M and a positive Hill coefficient (n = 1.25), suggesting tha t the N-myristoyl group confers some degree of cooperativity in Ca2+ bindin g, as seen previously in recoverin. Both the NMR and fluorescence spectra o f Frq1 exhibited very large Ca2+-dependent differences, indicating major co nformational changes induced upon Ca2+ binding. Nearly complete sequence-sp ecific NMR assignments were obtained for the entire carboxy-terminal domain (residues K100-I190). Assignments were made for 20% of the residues in the amino-terminal domain; unassigned residues exhibited very broad NMR signal s, most likely due to Frq1 dimerization. NMR chemical shifts and nuclear Ov erhauser effect (NOE) patterns of Ca2+-bound Frq1 were very similar to thos e of Ca2+-bound recoverin, suggesting that the overall structure of Frq1 re sembles that of recoverin. A model of the three-dimensional structure of Ca 2+-bound Frq1 is presented based on the NMR data and homology to recoverin. N-myristoylation of Frq1 had little or no effect on its NMR and fluorescen ce spectra, suggesting that the myristoyl moiety does not significantly alt er Frq1 structure. Correspondingly, the NMR chemical shifts for the myristo yl group in both Ca2+-free and Ca2+-bound myr-Frq1 were nearly identical to those of free myristate in solution, indicating that the fatty acyl chain is solvent-exposed and not sequestered within the hydrophobic core of the p rotein, unlike the myristoyl group in Ca2+-free recoverin. Subcellular frac tionation experiments showed that both the N-myristoyl group and Ca2+-bindi ng contribute to the ability of Frq1 to associate with membranes.