Ganglioside molecular species containing C18-and C20-sphingosine in mammalian nervous tissues and neuronal cell cultures

Gangliosides exist as a very complex mixture of species differing in both t he hydrophilic and hydrophobic moieties. They are particularly abundant in the central nervous system (CNS), where they have been associated with deve lopment and maturation of the brain, neuritogenesis, synaptic transmission, memory formation and synaptic aging. Today, many data suggest that some of the effects exerted by gangliosides are due to interactions with proteins that participate in the transduction of signals through the membrane in mem brane microdomains. A specific characteristic of CNS gangliosides is the st ructure of their long-chain base (LCB). In fact, considering all the mammal ian cell sphingolipids,,gangliosides, sulphatides, neutral glycosphingolipi ds, sphingomyelin and ceramides, it would seem that while the LCB with 18 c arbons is the main component of all sphingolipids, only CNS gangliosides co ntain significant amounts of LCB with 20 carbons. C18-Sphingosine is always present in cell gangliosides; the individual ganglioside species containin g C18-sphingosine increase during cell differentiation then remain constant during cell aging. Gangliosides containing C20-sphingosine are absent, or present only in traces, in undifferentiated cells but with the onset of cel l differentiation they appear, their content slowly but continuously increa sing throughout the life span. In this review we discuss the chemistry, phy sico-chemistry and metabolism of ganglioside species differing in LCB lengt h and introduce the hypothesis that the varying ratio between C18- and C20- gangliosides during CNS development and aging can be instrumental in modula ting membrane domain organisation and cell properties. (C) 2000 Elsevier Sc ience B.V. All rights reserved.