The turbidimetric study of latex particle agglutination: Assay of 2,4-dichlorophenoxyacetic acid

By the example of 2,4-dichlorophenoxyacetic acid (2,4-D) assay by the inhib ition of latex agglutination, new synthetic polymer microspheres for the co njugation with antibodies to 2,4-D and agglutinators based on ovalbumin and polyacrylamide were developed and characterized. The effect of various par ameters (the concentration of reagents, the type and the degree of modifica tion of the microsphere surface, and the nature of the carrier in the compo sition of the agglutinator) on the rate of agglutination and the maximal op tical absorption observed during the reaction were studied by turbidimetry. The optimal parameters were found for the assay of 2,4-D by the inhibition of latex agglutination with turbidimetric registration of the results.