Quantitation of phosphotyrosine signals in human prostate cell adhesion sites

A simple method is described for the quantitation of phosphotyrosine signal ing in human prostate cell cultures. The phosphotyrosine signals are observ ed by standard immunohistochemistry techniques, and the resulting digital i mages are analyzed using the Scion image software program. The signals with in the cell adhesion sites are quantitated using the density slice and part icle analysis features of the software. The immunohistochemistry results ar e compared with detection of phosphotyrosine signals using a standard Weste rn blotting procedure with whole cell lysates. The resulting data is conver ted into graphs using the Sigma Plot Program. This method is illustrated us ing damage-induced signaling within cell adhesion sites after a low dose of ionizing radiation.