Cytochrome c reductase immobilized on carbon paste electrode and its electrocatalytic effect on the reduction of cytochrome c

Carbon paste electrodes were modified with cytochrome c reductase enzyme. T he behavior of the electrodes was characterized by cyclic voltammetry. The cytochrome c reduction was investigated both on the enzyme modified carbon paste electrode and in a homogeneous system. In the first case, a significa nt electrocatalytic effect on the reduction of cytochrome c was round. The effect is supported by an increase of 4.3 fold of the peak current and a sh ift of the cathodic peak potential toward less cathodic values. Also, appar ent Michaelis constant values were calculated. Results from this work show that carbon paste electrode modified by the cyt ochrome c reductase represents a new interface that will permit the study o f electron transfer reactions involved in biodegradation processes.