Separation by Capillary Electrophoresis of C-Glycosylflavonoids in Passiflora sp extracts

The potential of Capillary Electrophoresis (CE) for separation of C-Glycosy lflavonoids has been examined. These compounds are found in Passiflora inca rnata L. and Passiflora coerulea L, and their quantitative evaluation is th e method for quality control of their extracts. Capillary zone electrophore sis (CZE) as well as micellar electrokinetic chromatography (MEKC) conditio ns were assayed. The optimum resolution of analytes was achieved within 12 min by using an electrolyte system of 50 mM sodium tetraborate, adjusted to pH 9.2 with NaOH and direct detection at 270 nm. Moreover, identification of several peaks was accomplished by repeating and comparing the migration times or electrophoretic mobilities and by spiking the sample solution with the pure compound prior to injection.