Detection of telomerase activity in peritoneal lavage fluid from patients with gastric cancer using immunomagnetic beads

Cytologic examination of peritoneal lavage fluid is a useful predictor of p eritoneal recurrence in gastric cancer. However, this technique is not over ly sensitive and requires special abilities in the cytologist. in this stud y, telomerase activity was used to detect free cancer cells in peritoneal l avage fluid from patients with gastric cancer. In the first part, 12 lavage -fluid samples obtained from 12 patients with gastric cancer were analysed using the conventional telomeric repeat amplification protocol (TRAP) assay . Three of five patients with early gastric cancer had positive telomerase activity. These false-positive results may have been due to lymphocyte cont amination. Furthermore. polymerase chain reaction inhibitors were also dete cted in the lavage-fluid samples. Therefore, we developed a novel method fo r elimination of haematopoietic cell and Tao polymerase inhibitors to incre ase the accuracy of the TRAP assay using immunomagnetic beads, which bind t o most normal and neoplastic human epithelial cells. Telomerase activity wa s found in 10 of 20 (50%) lavage-fluid samples from patients with serosal o r subserosal invasion. Cytologic examination was positive in nine of 20 (45 %) samples. Both the telomerase activity and cytology were negative in all 14 patients without serosal or subserosal invasion. These results suggest t hat the TRAP assay combined with immunomagnetic beads might be useful for d etection of tree cancer cells in the peritoneal space in gastric cancer wit hout the aid of an experienced cytologist. (C) 2000 Cancer Research Campaig n.