Aims-To investigate the presence of soluble Fas ligand (sFasL) and soluble
Fas (sFas) in ocular fluid of patients with uveitis.
Methods-Samples of aqueous humour (AH, n=17), vitreous fluid (n=9), and ser
um (n=60) were collected from patients with uveitis which included Behcet's
disease, Vogt-Koyanagi-Harada disease, sarcoidosis, human T lymphotropic v
irus type 1 (HTLV-I) uveitis, sympathetic ophthalmia, HLA-B27 associated ac
ute anterior uveitis, and ocular toxoplasmosis. The AH of patients with age
related cataract without uveitis obtained during cataract surgery was used
as controls (n=20). The amounts of sFasL and sFas were measured by enzyme
linked immunosorbent assay.
Results-Significant amounts of sFasL were detected in AH of patients with a
ge related cataract (non-uveitis group). sFasL was also detected in AH of p
atients with uveitis, though the amounts were slightly lower than those in
the non-uveitis group. On the other hand, the levels of sFas in AH of patie
nts with uveitis were significantly higher than those in controls. As for t
he disease activity, the levels of sFasL and sFas in the vitreous fluid of
patients with active uveitis were significantly higher than those in inacti
ve uveitis. sFasL in the serum of healthy donors and patients with uveitis
was below detectable levels, except for patients with HTLV-I uveitis who ha
d significant amounts of sFasL in the serum. The levels of sFas in the seru
m of patients with Behcet's disease, sarcoidosis, and HTLV-I uveitis were s
ignificantly higher than those of healthy donors.
Conclusions-sFasL is present in the AH of non-uveitic eyes with age related
cataract. Intraocular levels of sFasL and sFas are significantly increased
in uveitis, particularly in active uveitis. These data suggest that intrao
cular sFasL and sFas may have a regulatory role in uveitis.