Outstanding stability of immobilized recombinant alpha(1 -> 3/4)-fucosyltransferases exploited in the synthesis of Lewis a and Lewis x trisaccharides

Authors
Auge, C Malleron, A Tahrat, H Marc, A Goergen, JL Cerutti, M Steelant, WFA Delannoy, P Lubineau, A
Citation
C. Auge et al., Outstanding stability of immobilized recombinant alpha(1 -> 3/4)-fucosyltransferases exploited in the synthesis of Lewis a and Lewis x trisaccharides, CHEM COMMUN, (20), 2000, pp. 2017-2018
Citations number
8
Categorie Soggetti
Chemistry
Journal title
CHEMICAL COMMUNICATIONS
ISSN journal
13597345 → ACNP
Issue
20
Year of publication
2000
Pages
2017 - 2018
Database
ISI
SICI code
1359-7345(2000):20<2017:OSOIRA>2.0.ZU;2-D
Abstract
Recombinant human alpha(1 --> 3/4)-fucosyltransferases (FucT-III) expressed in CHO cells or baculovirus-infected insect cells, immobilized on Ni2+-aga rose through a 6His tag, exhibit a marked stability, which was exploited in the synthesis of Lewis a and Lewis x trisaccharides.