Background: Transcobalamin is essential for the cellular internalization of
cobalamin. Methods to quantify the unsaturated protein are available, but
few attempts have been made to develop methods to quantify the sum of unsat
urated and cobalamin saturated transcobalamin.
Methods: gamma-Globulins from two polyclonal rabbit antibodies against reco
mbinant human transcobalamin were used as capture and detection antibodies,
and recombinant human transcobalamin was used as calibrator in an ELISA de
sign.
Results: The ELISA is specific for transcobalamin and has a detection limit
of <1.6 pmol/L. The imprecision (CV) is 4-6% for mean concentrations of 13
-70 pmol/L. The central 95% interval for serum from healthy blood donors (n
= 77) was similar to 600-1500 pmol/L and showed limited variation with age
and sex. No correlation was observed between the marker of acute phase rea
ction, C-reactive protein, and transcobalamin in plasma.
Conclusions: The ELISA measures total transcobalamin in serum and thus can
be used for measurement of transcobalamin in patients treated with cobalami
n. (C) 2000 American Association for Clinical Chemistry.