Detection of genes essential in specific niches by signature-tagged mutagenesis

Authors
Lehoux, DE Levesque, RC
Citation
De. Lehoux et Rc. Levesque, Detection of genes essential in specific niches by signature-tagged mutagenesis, CURR OPIN B, 11(5), 2000, pp. 434-439
Citations number
22
Categorie Soggetti
Microbiology
Journal title
CURRENT OPINION IN BIOTECHNOLOGY
ISSN journal
09581669 → ACNP
Volume
11
Issue
5
Year of publication
2000
Pages
434 - 439
Database
ISI
SICI code
0958-1669(200010)11:5<434:DOGEIS>2.0.ZU;2-W
Abstract
Variations of the signature-tagged mutagenesis (STM) technique are now poss ible and the method can be applied to most pathogens that have an STM-selec table phenotype in a host system. STM screening of 15,040 mutants from 11 b acterial species identified 323 in vivo attenuated mutants. As a genome-sca nning tool, STM will yield information about genes with unknown functions a s well as information crucial for understanding microbial pathogenesis.