Characterization of three hairy cell leukemia-derived cell lines (ESKOL, JOK-1, and Hair-M) by multiplex-FISH, comparative genomic hybridization, FISH, PRINS, and dideoxyPRINS

Hairy cell leukemia (HCL) is a chronic B-lymphocyte leukemia. Due to the pr oliferative inertia of the malignant cells, HCL-derived cell lines are an i mportant tool for studies on this disease. We have elaborated the karyotype s of three HCL-derived cell lines, ESKOL, JOK-1, and Hair-M, by combining a range of molecular cytogenetic techniques, including multiplex (multicolor ) fluorescence in situ hybridization (M-FISH), comparative genomic hybridiz ation (CGH), conventional FISH, primed in situ labeling (PRINS), and dideox y-PRINS. We found ESKOL to be monoclonal with a single chromosome aberratio n, der(7)t(3;7)(q26.3;q31 ). JOK-1 also appeared to be monoclonal, having t he karyotype 48,XY,der(4) t(1;4)(1pter-->p32::4qter-->pter), der(6)(6qter-- >p22::q12--> qter),+der(7)t(7;11)(7pter-->q21::11p15-->pter),der(8)t(5;8;12 ) (5pter-->p14::12p11.2-->p12::8q12-->q21::8?cen-->-->24.?2), der(14)t(8;14 )(8qter-->q24.?2::14q32.3-->pter),+20. These karyotypes differ from the ori ginal descriptions of ESKOL and JOK-1. The Hair-M cells analyzed by us were found to be peritetraploid with numerous chromosomal rearrangements. The c ell line was also found to be multiclonal. On this basis, we do not regard the Hair-M cell line to be suitable for HCL studies. Copyright (C) 2000 S, KargerAG, Basel.