B. Posteraro et al., Commercial systems for fluconazole susceptibility testing of yeasts: comparison with the broth microdilution method, DIAG MICR I, 38(1), 2000, pp. 29-36
Fluconazole susceptibility was tested in 100 clinical yeast isolates (65 Ca
ndida albicans, 13 C. glabrata, 8 C. tropicalis, 7 C. parapsilosis, 3 Sacch
aromyces cerevisiae, 1 each of C. krusei, C. lusitaniae, Cryptococcus neofo
rmans, Rhodotorula glutinis) and two control strains (Candida krusei ATCC(R
) 6258, C. parapsilosis ATCC(R) 22019) using broth microdilution (reference
method), disk diffusion, Etest strips, Sensititre YeastOne, Candifast, Int
egral System Yeasts. Using M27-A breakpoints, isolates were classified as s
usceptible (81%), susceptible-dose dependent or Resistant with broth diluti
on. Rates of concordance with the reference method were good for Sensititre
YeastOne, Etest and disc-diffusion (81.2%-94.7%) but very low for the Cand
ifast(R) (3.1%) and Integral System (16.6%), which classified most suscepti
ble isolates as resistant. Lack of standardisation (inoculum, medium compos
ition) and non-objective interpretation schemes may be the cause of their p
oor performance. Sensititre YeastOne, Etest and disc-diffusion are potentia
lly useful for fluconazole antifungal susceptibility testing of yeasts in c
linical laboratories. (C) 2000 Elsevier Science Inc. All rights reserved.