Prevalence of non-O157 : H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska

Authors
Fey, PD Wickert, RS Rupp, ME Safranek, TJ Hinrichs, SH
Citation
Pd. Fey et al., Prevalence of non-O157 : H7 shiga toxin-producing Escherichia coli in diarrheal stool samples from Nebraska, EM INFECT D, 6(5), 2000, pp. 530-533
Citations number
23
Categorie Soggetti
Clinical Immunolgy & Infectious Disease
Journal title
EMERGING INFECTIOUS DISEASES
ISSN journal
10806040 → ACNP
Volume
6
Issue
5
Year of publication
2000
Pages
530 - 533
Database
ISI
SICI code
1080-6040(200009/10)6:5<530:PON:HS>2.0.ZU;2-D
Abstract
We determined the prevalence of Shiga toxin-producing Escherichia coli(STEC ) in diarrheal stool samples from Nebraska by three methods: cefixime-tellu rite sorbitol MacConkey (CT-SMAC) culture, enterohemorrhagic E. coli (EHEC) enzyme immunoassay, and stx(1,2)polymerase chain reaction (PCR). Fourteen (4.2%) of 335 specimens were positive by at least one method (CT-SMAC cultu re [6 of 14], EHEC enzyme immunoassay [13 of 14], stx(1,2)PCR [14 of 14]). Six contained serogroup 0157, while non-0157 were as prevalent as 0157 sero groups.