Stage-specific apoptotic patterns during Drosophila oogenesis

Citation
Ip. Nezis et al., Stage-specific apoptotic patterns during Drosophila oogenesis, EUR J CELL, 79(9), 2000, pp. 610-620
Citations number
43
Categorie Soggetti
Cell & Developmental Biology
Journal title
EUROPEAN JOURNAL OF CELL BIOLOGY
ISSN journal
01719335 → ACNP
Volume
79
Issue
9
Year of publication
2000
Pages
610 - 620
Database
ISI
SICI code
0171-9335(200009)79:9<610:SAPDDO>2.0.ZU;2-K
Abstract
In the present study we demonstrate the existence of two apoptotic patterns in Drosophila nurse cells during oogenesis, One is developmentally regulat ed and normally occurs at stage 12 and the other is stage-specific and is s poradically observed at stages 7 and 8 of abnormally developed follicles, T he apoptotic manifestation of the first pattern begins at stage 11 and is m arked by a perinuclear rearrangement of the actin cytoskeleton and the deve lopment of extensive lobes and engulfments of the nurse cell nuclei located proximal to the oocyte, Consequently, at late stage 12 (12C), half of the nurse cell nuclei exhibit condensed chromatin, while at late stage 13 all t he nuclei have fragmented DNA, as it is clearly shown by TUNEL assay. Final ly, the apoptotic vesicles that are formed during stage 13, are phagocytose d by the neighboring follicle cells and at stage 14 the nurse cell nuclear remnants can be easily detected within the adjacent follicle cell phagosome s, In the second sporadic apoptotic pattern, all the nurse cell nuclei are highly condensed with fragmented DNA, accompanied by a completely disorgani zed actin cytoskeleton, When we induced apoptosis in Drosophila follicles t hrough an etoposide and staurosporine in vitro treatment, we observed a sim ilar pattern of stage-specific cell death at stages 7 and 8, These observat ions suggest a possible protective mechanism throughout Drosophila oogenesi s that results in apoptosis of abnormal, damaged or spontaneously mutated f ollicles before they reach maturity.