Serine base-exchange in rat liver nuclei

Citation
A. Dygas et al., Serine base-exchange in rat liver nuclei, FEBS LETTER, 482(3), 2000, pp. 205-208
Citations number
37
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
482
Issue
3
Year of publication
2000
Pages
205 - 208
Database
ISI
SICI code
0014-5793(20001006)482:3<205:SBIRLN>2.0.ZU;2-7
Abstract
It has been shown that the incorporation of [C-14]serine into phosphatidyls erine (PS) in isolated rat liver nuclei is intrinsic to this organelle as a ttested by marker enzyme activity. Serine incorporation into PS was the hig hest in nuclei depleted of the outer membrane of the nuclear envelope (nucl eoplasts) and negligible in the outer membrane. Trypsin treatment of nucleo plasts caused a strong inactivation of PS synthesis and only a moderate one of the NAD pyrophosphorylase activity, the marker enzyme of the inner nucl ear membrane. We suggest that the serine base-exchange enzyme is located in the inner membrane of the nuclear envelope and accessible from the peripla smic surface of this membrane. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.