Duplication of genes encoding non-clathrin coat protein gamma-COP in vertebrate, insect and plant evolution

Coatomer is a major component of COPI vesicles and consists of seven subuni ts, The gamma-COP subunit of the coatomer is believed to mediate the bindin g to the cytoplasmic dilysine motifs of membrane proteins. We characterized cDNAs for Copg genes encoding gamma-COP from mouse, zebrafish, Drosophila melanogaster and Bombyx mori. Two copies of Copg genes are present in verte brates and in B.mori. Phylogenetic analysis revealed that two paralogous ge nes had been derived from a single ancestral gene by duplication independen tly in vertebrates and in B. mori.Mouse Copg1 shelved ubiquitous expression with the highest level in testis, Zebrafish copg2 was biallelically expres sed in hybrid larvae in contrast to its mammalian ortholog expressed in a p arent-of-origin-specific manner. A phylogenetic analysis with partial plant cDNA sequences suggested that copg gene was also duplicated in the grass f amily (Poaceae), (C) 2000 Federation of European Biochemical Societies. Pub lished by Elsevier Science B.V. All rights reserved.