Analysis of dipeptidyl peptidase IV gene regulation in transgenic mice: DNA elements sufficient for promoter activity in the kidney, but not the intestine, reside on the proximal portion of the gene 5 '-flanking region

The dipeptidyl peptidase IV (DPPIV) gene encodes a brush border membrane ex opeptidase that is expressed in a tissue-restricted fashion. To examine the regulation of DPPIV transcription in various tissues in vivo we examined t he expression of DPPIV 5'-flanking region (promoter)-human growth hormone r eporter constructs in transgenic mice. These mice exhibited cell-type speci fic reporter expression in kidney, Surprisingly, how ever, only very low to non-detectable levels of reporter were found in small intestine. These res ults indicate that DNA elements sufficient for DPPIV expression in kidney, but not intestine, reside in the 5'-flanking region of the gene. (C) 2000 F ederation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.