Mm. Gherardi et al., Salmonella enteritidis temperature-sensitive mutants protect mice against challenge with virulent Salmonella strains of different serotypes, FEMS IM MED, 29(2), 2000, pp. 81-88
The protection conferred by temperature-sensitive mutants of Salmonella ent
eritidis against different wild-type Salmonella serotypes was investigated.
Oral immunization with the single temperature-sensitive mutant E/1/3 or wi
th a temperature-sensitive thymine-requiring double mutant (E/1/3T) conferr
ed: (i) significant protection against the homologous wild-type Salmonella
strains; (ii) significant cross-protection toward high challenge doses of S
. typhimurium. Significant antibody levels against homologous lipopolysacch
aride and against homologous and heterologous protein antigens were detecte
d in sera from immunized mice. Moreover, a wide range of protein antigens f
rom different Salmonella O serotypes were recognized by sera from immunized
animals. Besides, primed lymphocytes from E/1/3 immunized mice recognized
Salmonella antigens from different serotypes. Taken together, these results
indicate that temperature-sensitive mutants of S. enteritidis are good can
didates for the construction of live vaccines against Salmonella. (C) 2000
Federation of European Microbiological Societies. Published by Elsevier Sci
ence B.V. All rights reserved.