Optimisation of composition of media for the production of amylolytic enzymes by Thermomyces lanuginosus ATCC 34626

The composition of media for the production of amylolytic enzymes by Thermo myces lanuginosus was optimised in different ways. Effects of various carbo n and nitrogen sources were investigated. Thermomyces lanuginosus grown on starch, maltodextrin, dextrin, maltose, amylopectin, glucose and dextran su bstrates showed good alpha-amylase (92-125 U/mL) and glucoamylase (6-13 U/m L) activities. Among the tested nitrogen sources L-asparagine was the best one. The optimum pH of fermentation medium was found and fixed to 4.9 by us ing 100 mM citrate buffer for the production of amylolytic enzymes. Respons e Surface Method (RSM) was applied for searching the optimum concentration of components of media for amylolytic enzyme production. A second-order pol ynomial model was fitted at significance level 95 % (P<0.05) for both a-amy lase and glucoamylase. The developed composition of media was checked in re spect of the production of amylolytic enzymes. The concentration of L-aspar agine was adjusted to 0.75 %. The concentration of starch was set at 6.5 % for alpha-amylase and 2 % for glucoamylase.