F. Carriere et al., The specific activities of human digestive lipases measured from the in vivo and in vitro lipolysis of test meals, GASTROENTY, 119(4), 2000, pp. 949-960
Background & Aims: The lipolytic potential of digestive lipases in vivo has
always been deduced so far from their in vitro activities under nonphysiol
ogic conditions, In the present study, the specific activities of human gas
tric lipase (HGL) and pancreatic lipase (HPL) were measured on dietary trig
lycerides (TGs) during test meal lipolysis, Methods: Healthy human voluntee
rs ingested a liquid or solid meal, The specific activities of HGL and HPL
were estimated from the lipase and free fatty acid (FFA) outputs at the pos
tpyloric and duodenal levels, respectively. Based on the in vivo data, lipo
lysis was also performed in vitro by mixing the meal either with gastric ju
ice and subsequently with pancreatic juice and bile or with purified HGL an
d HPL, FFAs were measured by thin-layer chromatography, and the specific ac
tivities of HGL and HPL were expressed as micromoles of FFA per minute per
milligram of lipase, Results: In vitro, the specific activities on the liqu
id meal TGs were 32 (gastric juice) and 34 (pure lipase) mu mol.min(-1).mg(
-1) with HGL and 47 (pancreatic juice) and 43 (pure lipase) mu mol.min(-1).
mg(-1) with HPL. The specific activities on the solid meal TGs were 33 (gas
tric juice) and 32 (pure lipase) mu mol.min(-1).mg(-1) with HGL and 12 (pan
creatic juice) and 15 (pure lipase) mu mol.min(-1).mg(-1) with HPL, The in
vivo values obtained were in the same range, The secretory lipase outputs w
ere 21.6 +/- 14.5 mg HGL and 253.5 +/- 95.5 mg HPL with the liquid test mea
l and 15.2 +/- 5.1 mg HGL and 202.9 +/- 96.1 mg HPL with the solid test mea
l, Conclusions: The specific activities of HGL and HPL on meal TGs were muc
h lower than those measured in vitro under optimized assay conditions (1300
-8000). However, these low specific activities are enough for the meal TGs
to be completely lipolysed, given the amounts of HGL and HPL secreted durin
g a meal.