VALIDATION OF A CAPILLARY ELECTROPHORESIS PROCEDURE FOR THE DETERMINATION OF CALCIUM IN CALCIUM ACAMPROSATE

The quantitative aspects of a capillary electrophoresis (CE) technique for the determination of calcium in calcium acamprosate are reported. Separation was carried out on a 57-cm (50 cm to the detector)x75-mu m I.D. fused-silica capillary at a potential of 10 kV and 25 degrees C, using as electrolyte 10 mM imidazole containing 1 mM tetrabutylammoni um sulfate adjusted to pH 4.5 with sulfuric acid. Standard solutions o f calcium carbonate and test solutions of calcium acamprosate containi ng 10 ppm of Ca2+ and Mg2+ (internal standard) were injected hydrodyna mically for 3 s. Indirect UV detection was carried out at 214 nm. A sa tisfactory agreement was found between CE, complexometry and theoretic al content for determination in calcium acamprosate. Precision of CE o n different capillaries using an internal standard complies with the r equirements of quality control for the drug substance.