I. Miksik et al., MICROEMULSION ELECTROKINETIC CHROMATOGRAPHY OF DIPHENYLHYDRAZONES OF DICARBONYL SUGARS, Journal of chromatography, 772(1-2), 1997, pp. 297-303
Citations number
26
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A mixture of diphenylhydrazones of dicarbonyl sugars was separated by
microemulsion electrokinetic chromatography. Separations were carried
out with a laboratory-made capillary electrophoresis apparatus using a
n untreated fused-silica capillary [70 cm (effective length 65 cm)x50
mu m I.D.] at 20 kV; detection was at 220 nm. The microemulsion system
was composed of 5 mM berate buffer, pH 8.0 (89.27%, w/w), sodium dode
cyl sulphate (SDS; 3.31%, w/w), n-butanol (6.61%, w/w) and n-octanol (
0.81%, w/w). Separation by microemulsion electrokinetic chromatography
was significantly better then separation obtained by routine micellar
electrokinetic chromatography with SDS and was characterized by a muc
h higher efficiency (theoretical plates ranged from 100.10(3) to 254.1
0(3)). SDS-micellar electrokinetic chromatography resolved only one co
mpound from a joint peak of two sugars and from a cluster peak consist
ing of seven compounds. Applicability to the analysis of biological sa
mples was also demonstrated.