IDENTIFICATION OF ALPHA(1)-ACID GLYCOPROTEIN (OROSOMUCOID) IN HUMAN SYNOVIAL-FLUID BY CAPILLARY ELECTROPHORESIS

Capillary electrophoresis of human synovial fluid in a phosphate-borat e run buffer containing sodium dodecyl sulphate separates a hydrophili c glycoprotein, hyaluronan and a number of low-molecular-mass componen ts. The hydrophilic glycoprotein is identified as alpha(1)-acid glycop rotein (AGP), orosomucoid, by co-injection methods with human AGP and by reaction with neuraminidase which released N-acetylneuraminic acid. Finally, a sample of the glycoprotein was isolated by micropreparativ e capillary electrophoresis, examined by sodium dodecyl sulphate-polya crylamide gel electrophoresis methods and shown to give a positive rea ction with AGP antibodies. The peak due to AGP in the capillary electr ophoresis is broad and gives evidence for the presence of glycoforms.