Effects of endogenous ouabain on the development of hypertension in 1kle hypertensive rats

This study was designed to evaluate the role of endogenous ouabain (EO) in the development of hypertension in 1k1c tone kidney, one clip) hypertensive rats. First, the EO content of the serum of 1k1c hypertensive rats and nor mal Sprague-Dawley (SD) rats was detected by the enzyme linked immunosorben t assay method (ELISA), Second, blood pressure changes in the 1k1c rats wer e recorded directly after the 1k1c rats were injected randomly with anti-ou abain antibody, normal rabbit IgG, and normal saline, respectively. The res ults showed that EO levels in the serum of 1k1c hypertensive rats were sign ificantly higher than those of normal SD rats (2.25 +/- 0.92 mu g/l vs. 1.1 2 +/- 0.17 mu g/l, p < 0.01), and correlated significantly with systolic bl ood pressure (r = 0,59, p < 0.05). Anti-ouabain antibody was able to signif icantly decrease the blood pressure of 1k1c hypertensive rats in a dose-dep endent manner, while normal rabbit IgG or normal saline was not. These resu lts indicate that endogenous ouabain might play an important role in the de velopment of hypertension in 1k1c hypertensive rats.