M. Kakoki et al., Effects of vasodilatory antihypertensive agents on endothelial dysfunctionin rats with ischemic acute renal failure, HYPERTENS R, 23(5), 2000, pp. 527-533
Ischemic acute renal failure is associated with vascular endothelial dysfun
ction. We examined whether vasodilatory antihypertensive agents would impro
ve endothelial function in rats with ischemia/reperfusion renal injury. Rat
kidneys were isolated and perfused after clipping of the bilateral renal a
rteries for 45 min and reperfusion for 24 h, and renal perfusion pressure a
nd nitric oxide concentration in the venous effluent (chemiluminescence ass
ay) were monitored, Preischemic administration of celiprolol (a beta-blocke
r; 100 mg/kg p.o.), benidipine (a calcium channel blocker; 1 mg/kg p.o.), o
r imidapril (an angiotensin converting-enzyme inhibitor; 3 mg/kg p.o,) rest
ored endothelial function in rats subjected to acute renal ischemia (Delta
renal perfusion pressure [10(-8) M acetylcholine]: sham -42+/-3%, ischemia
-31+/-1%, ischemia +celiprolol -39+/-1%*, ischemia+benidipine -38+/-2%*, is
chemia+imidapril -42+/-2%*; *p<0.05 vs, ischemia), Serum urea nitrogen and
creatinine levels were also lower in the treated groups, Furthermore, ische
mia-induced decreases in the response to acetylcholine and renal excretory
function were smaller in SHR than in deoxycorticosterone-salt hypertensive
rats, in which endothelial damage was marked. These results suggest that pr
eischemic endothelial function may influence the degree of ischemic renal i
njury. Calcium channel blockers, converting enzyme inhibitors, and endothel
ial NO synthase-activating beta-blockers had beneficial effects on renovasc
ular endothelial dysfunction due to ischemia.