SMALL PEPTIDE LIBRARIES - COMBINATORIAL SPLIT-MIX SYNTHESIS FOLLOWED BY COMBINATORIAL AMINO-ACID-ANALYSIS OF SELECTED VARIANTS

Authors
WEINBERGER H LICHTE E GRIESINGER C KUTSCHER B
Citation
H. Weinberger et al., SMALL PEPTIDE LIBRARIES - COMBINATORIAL SPLIT-MIX SYNTHESIS FOLLOWED BY COMBINATORIAL AMINO-ACID-ANALYSIS OF SELECTED VARIANTS, Archiv der pharmazie, 330(4), 1997, pp. 109-111
Citations number
12
Categorie Soggetti
Chemistry,"Pharmacology & Pharmacy
Journal title
Archiv der pharmazie → ACNP
ISSN journal
03656233
Volume
330
Issue
4
Year of publication
1997
Pages
109 - 111
Database
ISI
SICI code
0365-6233(1997)330:4<109:SPL-CS>2.0.ZU;2-O
Abstract
Peptides from small combinatorial libraries, covalently attached to po lymeric TentaGel beads, can be directly sequenced using amino acid ana lysis. For libraries with restricted diversity, generated by the split -mix synthesis method, the amino acids on a selected single head ident ified by pre-column derivatization with o-phthaldialdehyde (OPA) corre late directly with the sequence of a given peptide. This is shown on a tripeptide (343 different compounds) and a tetrapeptide (4096 differe nt compounds) library. This method allows for rapid peptide sequence d etermination without relying on complex encoding strategies.