P. Krumbiegel et Dm. Muller, Helicobacter pylori in vivo diagnosis after food intake: Results of simultaneous [N-15]urea urine and [C-13]acetate breath tests, ISOT ENV H, 36(2), 2000, pp. 99-110
The protocols for C-13 and N-15 H. pylori tests stipulate that the diagnost
ic agent should be taken on an empty stomach. It is presumed that food inta
ke prior to the tests leads to less reliable test results due to a prolonga
tion of the gastric residence time of the diagnostic agent urea. This might
allow the bacteria to sprit a higher proportion of urea, resulting in an i
ncreased number of false positives. 12 probands received 150 mg [N-15]urea
and 75 mg sodium [C-13]acetate in 75 ml orange juice as a test drink. [N-15
]Urea served as an agent to diagnose gastric H, pylori colonization. The 15
N tests were evaluated using a urine sample of the second hour after test s
tart. [C-13]Acetate served as a marker of the gastric emptying of water-sol
uble food including the urea under the influence of food intake. Breath air
samples were taken to calculate the gastric emptying half life (EHL) and t
he apparent resorption time (RT) of the urea. The double tests were carried
out four times within four weeks using identical test protocols but differ
ent standardized time periods of pretest fasting: overnight, two hours prio
r to test, one hour prior to test, and no fasting at ail. The food intake a
mount was standardized.
Five probands testing positive in the overnight fasting test were also foun
d to be positive in the other test variants with more or less empty stomach
s. Seven other probands testing negative after overnight fasting tested neg
ative in the other test variants as well.
It is concluded that food intake prior to the test drink does not have much
of an influence on the gastric residence time of urea and so on the qualit
ative H. pylori test results. Due to identical behaviour of [C-13]urea and
[N-15]urea in the stomach, this influence is believed to be independent on
the labelling isotope. For survey purpose, no fasting conditions are requir
ed for the H. pylori tests.