Luteinizing hormone-releasing hormone induces nuclear factor kappa B-activation and inhibits apoptosis in ovarian cancer cells

More than 80% of human ovarian cancers express LHRH and its receptor as par t of a negative autocrine mechanism of growth control. This study was condu cted to investigate whether LHRH affects apoptosis in ovarian cancer. EFO-2 1 and EFO-27 ovarian cancer cells were treated with LHRH agonist Triptoreli n or with cytotoxic agent Doxorubicin in the absence or presence of Triptor elin. Apoptotic cells were quantified by flow cytometry. Expression of nucl ear factor kappa B (NF kappa B) was assessed by RT-PCR and immunoblotting. For determination of Triptorelin-induced NF kappa B activation, cells were transfected with a NF kappa B-secreted alkaline phosphatase reporter gene p lasmid (pNF kappa B-SEAP) and cultured for 96 h with or without Triptorelin . The causal relation between Triptorelin-induced NF kappa B activation and Triptorelin-induced protection against apoptosis was investigated using SN 50, an inhibitor for nuclear translocation of activated NF kappa B. Apoptos is induction by Triptorelin was never observed. Treatment with Doxorubicin (1 nmol/L) for 72 h increased the percentage of apoptotic cells in EFO-21 a nd EFO-27 ovarian cancer cell lines to 31% or 34%, respectively. In culture s treated simultaneously with Triptorelin (100 nmol/L), the percentage of a poptotic cells was reduced significantly, to 17% or 18%, respectively (P < 0.001). RT-PCR and immunoblotting experiments showed that NF kappa B subuni ts p50 and p65 were expressed by ovarian cancer cell lines EFO-21 and EFO-2 7. When EFO-21 or EFO-27 cells were transfected with pNF kappa B-SEAP and s ubsequently treated with Triptorelin (100 nmol/L), NF kappa B-induced SEAP expression increased 5.3-fold or 4.7-fold, respectively (P < 0.001). Tripto relin-induced reduction of Doxorubicin-induced apoptosis was blocked by SN5 0-mediated inhibition of NF kappa B translocation into the nucleus. We conc lude that LHRH induces activation of NF kappa B and thus reduces Doxorubici n-induced apoptosis in human ovarian cancer cells. This possibility to prot ect ovarian cancer cells from programmed cell death is an important feature in LHRH signaling in ovarian tumors, apart from the inhibitory interferenc e with the mitogenic pathway.