Identification of five different Patr class I molecules that bind HLA supertype peptides and definition of their peptide binding motifs

We have sequenced the Pan troglodytes class I (Patr) molecules from three c ommon chimpanzees and expressed them as single molecules in a class I-defic ient cell line. These lines were utilized to obtain purified class I molecu les to define the peptide binding motifs associated with five different Pat r molecules, Based on these experiments, as well, as analysis of the predic ted structure of the B and F polymorphic MHC pockets, we classified five Pa tr molecules (Patr-A*0101, Patr-B*0901, Patr-B*0701, Patr-A*0602, and Patr- B*1301) within previously defined supertype specificities associated with H LA class I molecules (HLA-A3, -B7, -A1, and -A24 supertypes), The overlap i n the binding repertoire between specific HLA and Patr class I molecules wa s in the range of 33 to 92%, depending on the particular Patr molecule as a ssessed by the binding of HIV-, hepatitis B virus-, and hepatitis C virus-d erived epitopes, Finally, live cell binding assays of nine chimpanzee-deriv ed B cell lines demonstrated that HLA supertype peptides bound to Patr clas s I molecules with frequencies in the 20-50% range.