Possible bases of pseudoparasitism in Spodoptera littoralis larvae stung by Microplitis rufiventris

The effects of host age and parasitoid female age on the occurrence of 'Pse udoparasitism', using the Spodoptera littoralis-Microplitis rufiventris hos t-parasitoid system were investigated. The first four larval instars of the host are not equally suitable for parasitoid development. The proportion o f pseudoparasitized hosts significantly increases when: (1) the age of the female parasitoid increases; (2) oviposition occurs mostly in fourth instar larvae; (3) a later age of the host instar is used; (4) the mandibles of t he newly hatched parasitoid larvae mistakenly attack host interior organs ( e.g. Malpighian tubules); and (5) an imperfect growth pattern of teratocyte s occurs. The reluctance of female wasps to parasitize fourth instar host l arvae is not due to the thickness of host cuticle but possibly due to the u nfavourable physiological state of the host larvae. The age of host larvae at the time of parasitization may influence the adverse effects of parasito id factors (e.g. polydnavirus, venom and teratocytes) on the growth of host larvae. It is suggested that females of M. rufiventris are able to determi ne the suitability of a potential host instar for the development of their offspring. The cell diameter of M. rufiventris teratocytes increases with i ncreasing age of host larvae at the time of oviposition. The association wi thin the host of living parasitoid larvae and functional teratocytes may be important for the survival of each other and consequently for successful p arasitism. (C) 2000 Elsevier Science Ltd. All rights reserved.