Indirect high performance liquid chromatographic enantioseparation of racemic amino alcohols with 1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate as derivatizing agent

Synthetically and pharmaceutically interesting racemic amino alcohols with two adjacent chiral centres were analysed by means of indirect high perform ance liquid chromatography. For resolution of the enantiomers, the recently developed chiral derivatizing agent (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl )-2-propyl isothiocyanate ((R,R)-DANI) was applied. The diastereomeric thio ureas produced after derivatization were separated under reversed-phase con ditions. Of the organic modifiers applied in the eluent, methanol proved mu ch more effective than acetonitrile.