Studies of liquid adsorption chromatography process with mixed mobile phases

Description of chromatographic retention mechanism in the systems with tern ary mobile phase is very complex. Designation of the effect of individual m obile phase components on this process is difficult. The number of moles (o r molecules) of less polar solvents of mobile phase referring to one mole ( or molecule) of polar solvent must be taken into account. In this connectio n, a new method of experimental data presentation was proposed. The associa tion effects and changes of surface phase composition were analysed. Experi mental data are presented as relationships R-M vs. (x(x)/x(2))(3) and R-M v s. (x(1)/x(3))(3). The influence of a chromatographed substance on the rete ntion process was also analysed. The relationships mentioned above enable t he effect of individual solvent on the chromatographic process.